Abstract

Lactobacillus rhamnosus GR-1 is a probiotic that has been shown to reduce the risk of urogenital problems and urinary tract infections. Rice pudding is a popular gluten-free dairy product, and could be a vehicle to deliver L. rhamnosus GR-1 to a broader population. The purpose of this study was to investigate the growth and viability of L. rhamnosus GR-1 in six fermented rice pudding samples, each one supplemented with one type of prebiotic (short-chain inulin-2% w/w, 4% w/w; long-chain inulin-2% w/w, 4% w/w) and oat-0.5% w/w, 1% w/w, along with control, over a 21-day storage period. The objective was to determine if the supplementation would have a positive effect on the microbial viability of L. rhamnosus GR-1, and to evaluate the sensory properties of the samples. All of the samples had viable levels of L. rhamnosus GR-1. Bacterial counts were at least 1 × 108 CFU/mL over the 21-day storage period. The probiotic rice pudding sample supplemented with 4% w/w short-chain inulin had the highest hedonic score for flavour, sweetness, texture, and overall acceptability. This study shows that the addition of short-chain inulin, long-chain inulin, and oat had no adverse supplementation effects on the viability of L. Rhamnosus GR-1. There is the potential for the production of a novel functional food.

Highlights

  • Probiotics are live non-pathogenic bacteria which benefit health when ingested [1]

  • The addition of long-chain inulin, short-chain inulin, and oat did not decrease the viability and survival of the bacteria when compared to the control sample (Table 1)

  • The results showed no adverse effects of short-chain inulin, long-chain inulin, or oat on the viability of L. rhamnosus GR-1

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Summary

Introduction

Probiotics are live non-pathogenic bacteria which benefit health when ingested [1]. Therapeutic benefits can vary according to specific species and strain. The most frequently used types of probiotics are Lactobacillus and Bifidobacterium species [2]. Lactobacillus rhamnosus GR-1 is bile-resistant and survives the passage through the gastrointestinal tract [3,4]. It has been found to relieve urogenital problems and urinary tract infections by colonizing intestinal and urogenital cells [5,6,7]. Most probiotics are available as over-the-counter items, and as fermented dairy products, such as yogurt and kefir [1,2]

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