Lactobacillus plantarum Surface-Displayed ASFV (p14.5) Can Stimulate Immune Responses in Mice.

Quntao Huang,Kaidian Yang,Tingting Sun,Di Zhang,Junhong Wang,Tianming Niu,Junhong Xin,Guilian Yang,Junfu Bao,Hui Niu,Nan Li,Chunfeng Wang,Xize Feng,Ying Wang,Dandan Zhao,Boshi Zou,Yuxin Jiang,Xin Wang

doi:10.3390/vaccines10030355

Quntao Huang, Kaidian Yang + Show 16 more

Open Access

https://doi.org/10.3390/vaccines10030355

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Journal: Vaccines	Publication Date: Feb 24, 2022
Citations: 3	License type: CC BY 4.0

Affiliation: Jilin Agricultural University

Abstract

African Swine Fever Virus (ASFV) has spread worldwide, and the lack of vaccines severely negatively impacts the pig industry. In this study, the p14.5 protein encoded by ASFV was used as the antigen, and the p14.5 gene was expressed in vitro using the Lactobacillus expression system. Three new functionally recombinant Lactobacillus plantarum (L. plantarum) were constructed and the expressions of the p14.5 protein, p14.5-IL-33-Mus fusion protein and CTA1-p14.5-D-D fusion protein were successfully detected using Western blot analysis. After oral immunization of SPF mice with recombinant L. plantarum, flow cytometry and ELISA were performed to detect the differentiation and maturity of T lymphocytes, B lymphocytes and DCs of the mice, which were higher than those of the control group. Specific antibodies were produced. The immunogenicity of the adjuvant group was stronger than that of the single antigen group, and the IL-33 adjuvant effect was stronger than that of the CTA1-DD adjuvant.

Highlights

African Swine Fever (ASF) is an acute, febrile and highly contagious pig disease caused by the African Swine Fever Virus (ASFV)
The results showed that feeding recombinant L. plantarum had an effect on improving the immunity of mice, promoting the differentiation and maturation of T and B lymphocytes and DC cells, and stimulating T cells to produce relatively high amounts of cytokines
The results showed that the target band was consistent with the expected size, which proved that the recombinant L. plantarum successfully expressed the foreign protein

Summary

Introduction

African Swine Fever (ASF) is an acute, febrile and highly contagious pig disease caused by the African Swine Fever Virus (ASFV). ASF manifests as high fever, skin congestion, abortion, edema and organ bleeding. As a late-expressing protein, p14.5 is a key virulence factor of ASFV. It can bind interferon regulatory factor 3 (IRF3) to block its recruitment and plays an important role in inhibiting IRF3 phosphorylation and interferon production. The p14.5 protein can cause a humoral response and is synthesized in the late stage of infection and is located in the virus factory. It is a protein necessary for the transfer of virus particles from the virus factory to the plasma membrane and can combine with the

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