Lactobacillus plantarum MA2 Ameliorates Methionine and Choline-Deficient Diet Induced Non-Alcoholic Fatty Liver Disease in Rats by Improving the Intestinal Microecology and Mucosal Barrier.

Yanping Wang,Haoran Li,Weitao Geng,Jinju Wang,Yang Zhang,Jingnan Yang

doi:10.3390/foods10123126

Yanping Wang, Haoran Li + Show 4 more

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https://doi.org/10.3390/foods10123126

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Abstract

Non-alcoholic fatty liver disease (NAFLD) has become a highly concerned health issue in modern society. Due to the attentions of probiotics in the prevention of NAFLD, it is necessary to further clarify their roles. In this study, the methionine and choline-deficient (MCD) diet induced NAFLD rats model were constructed and treated with strain L. plantarum MA2 by intragastric administration once a day at a dose of 1 × 108 cfu/g.bw. After 56 days of the therapeutic intervention, the lipid metabolism and the liver pathological damage of the NAFLD rats were significantly improved. The content of total cholesterol (TC) and total triglyceride (TG) in serum were significantly lower than that in the NAFLD group (p < 0.05). Meanwhile, the intestinal mucosal barrier and the structure of intestinal microbiota were also improved. The villi length and the expression of claudin-1 was significantly higher than that in the NAFLD group (p < 0.05). Then, by detecting the content of LPS in the serum and the LPS-TLR4 pathway in the liver, we can conclude that Lactobacillus plantarum MA2 could reduce the LPS by regulating the gut microecology, thereby inhibit the activation of LPS-TLR4 and it downstream inflammatory signaling pathways. Therefore, our studies on rats showed that L. plantarum MA2 has the potential application in the alleviation of NAFLD. Moreover, based on the application of the strain in food industry, this study is of great significance to the development of new therapeutic strategy for NAFLD.

Highlights

Non-alcoholic fatty liver disease (NAFLD) covers a wide range of liver diseases from hepatic steatosis to non-alcoholic steatohepatitis (NASH) [1]
The analysis showed that compared with the normal group, the steatosis score, the lobular inflammation score and the ballooning score in NAFLD group reached 2.78 ± 0.44, 1.67 ± 0.5 and 2.00 ± 0, respectively (p < 0.05)
We demonstrated that the probiotic L. plantarum MA2 can interfere with

Summary

Introduction

Non-alcoholic fatty liver disease (NAFLD) covers a wide range of liver diseases from hepatic steatosis to non-alcoholic steatohepatitis (NASH) [1]. With the change of people’s lifestyle and diet structure, NAFLD has become the most widely distributed chronic liver disease in the world [2,3]. It is reported that the incidence of NAFLD in overweight and obese people is as high as 57% and 98% respectively. Due to the increasing incidence rate, NAFLD has become a highly concerned health issue in modern society [4]. NAFLD is a complex disease, which may be caused by many factors, including genetics, diet and intestinal microbiota [5,6,7,8]. In 1998, Day and James proposed the “two-hit”

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