Abstract
Obesity is a major health problem and is known to be closely associated with metabolic diseases. Abnormal hepatic accumulation of fat causes fatty liver or hepatic steatosis, and long-term consumption of a high-fat diet is known to be a key obesity-causing factor. Recent studies have demonstrated that probiotics such as Lactobacillus strains, exert an anti-obesity effect by regulating adipogenesis. However, it is still unknown how the consumption of probiotics can reduce abdominal fat volume by regulating the hepatic expression of lipogenic genes. Therefore, we evaluated the effect of long-term ingestion of L. plantarum LMT1-48 on the expression of lipogenic genes in high-fat diet (HFD)-fed mice. We observed that treatment of 3T3-L1 adipocytes with L. plantarum LMT1-48 extract inhibited their differentiation and lipid accumulation by downregulating lipogenic genes, namely, PPARγ, C/EBPα, FAS, and FABP4. Interestingly, administration of L. plantarum LMT1-48 reduced liver weight and liver triglycerides concurrently with the downregulation of the lipogenic genes PPARγ, HSL, SCD-1, and FAT/CD36 in the liver, resulting in the reduction of body weight and fat volume in HFD-fed obese mice. Notably, we also observed that the administration of at least 106 CFU of L. plantarum LMT1-48 significantly lowered body weight and abdominal fat volume in modified diet-fed mouse models. Collectively, these data suggest that L. plantarum LMT1-48 is a potential healthy food for obese people.
Highlights
Obesity is a major health problem and is known to be closely associated with metabolic diseases
Obesity is closely associated with liver abnormalities, including nonalcoholic fatty liver disease (NAFLD) and steatohepatitis[2,30]
Several studies have demonstrated that liver weight, liver triglycerides, and expression of lipogenic genes are closely associated with obesity[2,30], and the administration of lactic acid bacteria (LAB) inhibits the synthesis[32] and accumulation[33] of fats in high-fat diet (HFD)-induced obese mice
Summary
Obesity is a major health problem and is known to be closely associated with metabolic diseases. Recent studies have demonstrated that probiotics such as Lactobacillus strains, exert an anti-obesity effect by regulating adipogenesis It is still unknown how the consumption of probiotics can reduce abdominal fat volume by regulating the hepatic expression of lipogenic genes. We evaluated the effect of long-term ingestion of L. plantarum LMT1-48 on the expression of lipogenic genes in high-fat diet (HFD)-fed mice. Lipogenic genes are the key regulators of the synthesis[23] and accumulation[24] of fatty acids and differentiation of adipocytes[25] It is still unknown how the ingestion of probiotics can reduce abdominal fat volume by regulating the hepatic expression of lipogenic genes. We assessed the functional effects of long-term ingestion of L. plantarum LMT1-48 by evaluating the expression of lipogenic genes in the livers of high-fat diet (HFD)-fed mice
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