Abstract
Studies showed that specific probiotics provide therapeutic benefits in inflammatory bowel disease. In vitro evidence suggested that Lactobacillus paracasei also called ST11 (CNCM I-2116) is a potent strain with immune modulation properties. However, little is known about its capacity to alleviate inflammatory symptoms in vivo In this context, the main objective of this study was to investigate the role of ST11 on intestinal inflammation using the adoptive transfer mouse model of experimental colitis. Rag2−/− recipient mice were fed with ST11 (109 CFU/day)a month prior toinduce colitis by adoptive transfer of naive T cells. One month later, in clear contrast to nonfed mice, weight loss was significantly reduced by 50% in ST11-fed mice. Further analysis of colon specimens revealed a significant reduction neutrophil infiltration and mucosal expression of IL1β, IL-6, and IL12 proinflammatory cytokines, whereas no consistent differences in expression of antibacterial peptides or tight junction proteins were observed between PBS and ST11-fed mice. All together, our results demonstrate that oral administration of ST11 was safe and had a significant preventive effect on colitis. We conclude that probiotics such as Lactobacillus paracasei harbor worthwhile in vivo immunomodulatory properties to prevent intestinal inflammation by nutritional approaches.
Highlights
Probiotics are defined as live microbial food supplements that when ingested can survive gastrointestinal tract and exert positive influence on host health
Little is known about its capacity to alleviate inflammatory symptoms in vivo In this context, the main objective of this study was to investigate the role of ST11 on intestinal inflammation using the adoptive transfer mouse model of experimental colitis
We conclude that probiotics such as Lactobacillus paracasei harbor worthwhile in vivo immunomodulatory properties to prevent intestinal inflammation by nutritional approaches
Summary
Probiotics are defined as live microbial food supplements that when ingested can survive gastrointestinal tract and exert positive influence on host health. Many mechanisms have been reported to explain probiotic actions such as antagonism against intestinal pathogens, enhancement of mucosal barrier activity, or modulation of host’s immune functions as recently reviewed in [1]. The two major forms of IBD are Crohn’s disease and ulcerative colitis. Genetic predispositions as well as environmental factors such as diet or composition and activity of intestinal microbiota have been implicated in IBD pathogenesis [2]. Experimental colitis induced by adoptive transfer (ECIBAT) of naıve T cells in lymphopenic mice is an established animal model for IBD sharing a number of clinical, genetic, and immunological features with the human disease [3, 4]. ECIBAT is considered as one of the most relevant models to study IBD pathogenesis or to design and evaluate therapies
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