Abstract
Inflammatory bowel diseases (IBD), mainly comprising ulcerative colitis (UC) and Crohn's Disease, are most often a polygenic disorder with contributions from the intestinal microbiome, defects in barrier function, and dysregulated host responses to microbial stimulation. Strategies that target the microbiota have emerged as potential therapies and, of these, probiotics have gained the greatest attention. Herein, we isolated a strain of Lactobacillus paracasei R3 (L.p R3) with strong biofilm formation ability from infant feces. Interestingly, we also found L.p R3 strain can ameliorate the general symptoms of murine colitis, alleviate inflammatory cell infiltration and inhibit Th17 while promote Treg function in murine dextran sulfate sodium (DSS)-induced colitis. Overall, this study suggested that L.p R3 strain significantly improves the symptoms and the pathological damage of mice with colitis and influences the immune function by regulating Th17/Treg cell balance in DSS-induced colitis in mice.
Highlights
Inflammatory Bowel Disease (IBD) is a multifactorial chronic disease and mainly includes ulcerative colitis (UC) and Crohn’s disease
The survival rate of L.p R3 reached (58.76 ± 1.32)% and (43.08 ± 0.27)% after 3 h in PH 2.0 artificial gastric juice and intestinal fluids treatment (Fig. 1B). These results indicate that L.p R3 has good tolerance to gastrointestinal fluid and can survive and maintain activity under adverse gastrointestinal conditions
Lactobacillus paracasei (L.p) R3 strain alleviates inflammatory cell infiltration in murine dextran sulfate sodium (DSS)‐induced colitis We studied the colon of experimental mice and the results were shown in Fig. 4C, E
Summary
Inflammatory Bowel Disease (IBD) is a multifactorial chronic disease and mainly includes ulcerative colitis (UC) and Crohn’s disease. It is usually a polygenic disease caused by genetic make-up, barrier function, gut microbiome composition, environmental factor, and mucosal immune response [1]. From discovery to clinical application, probiotics have been developed and explored for many years. The category and mechanism of probiotics are gradually clear, and the clinical application is more and more extensive, and their functions mainly include intestinal protection, immune regulation, antibacterial effect, nutrition, antitumor, protecting liver, reducing blood lipid and so on [3,4,5,6,7,8,9]. Intestinal probiotics, as a kind of active microorganisms, need to be colonized in human intestinal epithelial cells, and communicate with the intestinal mucosa layer to play an important role in the metabolic, immune and intestinal health protection function of host individuals [3].
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