Lactobacillus Mediated Synthesis of Silver Oxide Nanoparticles

Zuzer H Dhoondia,Hemlatta Chakraborty

doi:10.5772/55741

Abstract

The ability of prokaryotic microorganisms to reduce the inorganic metals has opened up an exciting eco-friendly approach towards the development of natural ‘nano-factories’. However, a number of issues have to be addressed from the nanotechnological and microbiological point of view before such a biosynthesis approach can compete with the existing physical and chemical methods. This report investigates the synthesis of silver oxide nanoparticles using Lactobacillus mindensis, isolated using fixer solution from an X-ray photographic laboratory. Nanoparticles obtained were characterized by means of UV-vis spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction (XRD). The UV-vis spectrum shows the absorbance maximum at 430 nm, which is a characteristic of surface plasmon resonance of silver. Further, the presence of stable nanoparticles in the range of 2–20 nm was determined using TEM analysis. Silver nanoparticles in the form of silver oxide were confirmed in the XRD study. In conclusion, Lactobacillus mindensis serves as a promising candidate in the quest to synthesize silver oxide nanoparticles through green chemistry.

Highlights

Nanotechnology involves the study of structures at 1‐ 100 nanometres which possess novel properties and functions attributable to their small size [1]
The present study investigates biological synthesis of silver oxide nanoparticles using Lactobacillus mindensis isolated from fixer solution and presents an insight on the possible mechanism involved in the synthesis
Silver resistant bacterial species obtained from fixer solution of an X‐ray photographic laboratory were screened for the ability to produce silver nanoparticles

Summary

Introduction

Nanotechnology involves the study of structures at 1‐ 100 nanometres (nm) which possess novel properties and functions attributable to their small size [1]. When the colloidal particles are much smaller than the wavelength of visible light, the solutions have a yellow colour with an intense band in the 380–400 nm range and other less intense or smaller bands at a longer wavelength in the absorption spectrum. This band is attributed to collective excitation of the electron gas in the particles, with a periodic change in electron density at the surface (surface plasmon absorption) [5]

Methods

Results

Conclusion