Abstract
The probiotic Lactobacillus gasseri SBT2055 (LG2055) has anti-obesity effects. Obesity is closely correlated with inflammation in adipose tissue, and maintaining adipose tissue in a less-inflamed state requires intestinal integrity or a barrier function to protect the intestine from the disruption that can be caused by a high-fat diet (HFD). Here, we examined the anti-inflammatory and intestinal barrier-protecting effects of LG2055 in C57BL/6 mice fed a normal-fat diet (NFD), HFD, or the HFD containing LG2055 (HFD-LG) for 21 weeks. HFD-LG intake significantly prevented HFD-induced increases in body weight, visceral fat mass, and the ratio of inflammatory-type macrophages to anti-inflammatory ones in adipose tissue. Mice fed the HFD showed higher intestinal permeability to a fluorescent dextran administered by oral administration and an elevated concentration of antibodies specific to lipopolysaccharides (LPS) in the blood compared with those fed the NFD, suggesting an increased penetration of the gut contents into the systemic circulation. These elevations of intestinal permeability and anti-LPS antibody levels were significantly suppressed in mice fed the HFD-LG. Moreover, treatment with LG2055 cells suppressed an increase in the cytokine-induced permeability of Caco-2 cell monolayers. These results suggest that LG2055 improves the intestinal integrity, reducing the entry of inflammatory substances like LPS from the intestine, which may lead to decreased inflammation in adipose tissue.
Highlights
The probiotic Lactobacillus gasseri SBT2055 (LG2055) has anti-obesity effects
Despite a smaller intake quantity in the high-fat diet (HFD) and HFD containing LG2055 (HFD-LG) groups than in the normal-fat diet (NFD) group, energy intake was greater in the HFD and HFD-LG groups than in the NFD group, with no significant differences in either diet or energy intake between the HFD and HFD-LG groups
Compared with the HFD group, the HFD-LG group showed a significantly larger proportion of antiinflammatory alternatively activated macrophages (M2) macrophages (Fig. 1(c)) and a significantly lower classically activated macrophages (M1):M2 ratio (Fig. 1(d)), suggesting that the intake of the HFD-LG ameliorates an HFD-induced disposition in macrophage composition to a pro-inflammatory status
Summary
The probiotic Lactobacillus gasseri SBT2055 (LG2055) has anti-obesity effects. Obesity is closely correlated with inflammation in adipose tissue, and maintaining adipose tissue in a less-inflamed state requires intestinal integrity or a barrier function to protect the intestine from the disruption that can be caused by a high-fat diet (HFD). Mice fed the HFD showed higher intestinal permeability to a fluorescent dextran administered by oral administration and an elevated concentration of antibodies specific to lipopolysaccharides (LPS) in the blood compared with those fed the NFD, suggesting an increased penetration of the gut contents into the systemic circulation These elevations of intestinal permeability and anti-LPS antibody levels were significantly suppressed in mice fed the HFD-LG. Cani et al reported that a high-fat diet or an obese state disrupted the intestinal barrier and increased LPS levels in the blood[4] They demonstrated that mice administered LPS exhibited fat pad weight gain, inflammation in visceral adipose tissue and insulin resistance, all of which are common in obesity[3].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
