Abstract
Intestinal microbial interactions with the host epithelium have important roles in host health. Our previous data have suggested that Lactobacillus gasseri LA39 is the predominant intestinal Lactobacillus in weaned piglets. However, the regulatory role of L. gasseri LA39 in the intestinal epithelial protein expression in piglets remains unclear. In the present study, we conducted comparative proteomics approach to investigate the intestinal epithelial protein profile alteration caused by L. gasseri LA39 in piglets. The expressions of 15 proteins significantly increased, whereas the expressions of 13 proteins significantly decreased in the IPEC-J2 cells upon L. gasseri LA39 treatment. Bioinformatics analyses, including COG function annotation, GO annotation, and KEGG pathway analysis for the differentially expressed proteins revealed that the oxidative phosphorylation (OXPHOS) pathway in IPEC-J2 cells was significantly activated by L. gasseri LA39 treatment. Further data indicated that two differentially expressed proteins UQCRC2 and TCIRG1, associated with the OXPHOS pathway, and cellular ATP levels in IPEC-J2 cells were significantly up-regulated by L. gasseri LA39 treatment. Importantly, the in vivo data indicated that oral gavage of L. gasseri LA39 significantly increased the expression of UQCRC2 and TCIRG1 and the cellular ATP levels in the intestinal epithelial cells of weaned piglets. Our results, both in vitro and in vivo, reveal that L. gasseri LA39 activates the OXPHOS pathway and increases the energy production in porcine intestinal epithelial cells. These findings suggest that L. gasseri LA39 may be a potential probiotics candidate for intestinal energy production promotion and confers health-promoting functions in mammals.
Highlights
Growing evidence has suggested that intestinal microbes have critical roles in intestinal homeostasis and host health (Sommer and Backhed, 2013)
We used the isobaric tags for relative and absolute quantification (iTRAQ) strategy, where live L. gasseri LA39 were added into the IPEC-J2 cell medium for 4 h at 100:1 ratio to compare the intestinal epithelial cell protein profiles between the L. gasseri LA39 (LG) group and the control (Ctrl) group in the co-culture assay
The results demonstrated that the expression levels of UQCRC2 and TCIRG1 were significantly up-regulated upon L. gasseri LA39 treatment, which was consistent with the results of the iTRAQ analysis (Figure 5C)
Summary
Growing evidence has suggested that intestinal microbes have critical roles in intestinal homeostasis and host health (Sommer and Backhed, 2013). Intestinal microbes have been found to function in host immune defense system maturation (Ivanov et al, 2009), intestinal epithelium differentiation (Sommer and Backhed, 2013), and nutrients digestion (Backhed et al, 2007). L. gasseri LA39 Activates OXPHOS Pathway intestinal microbial dysbiosis can cause host gastrointestinal diseases (Borody and Khoruts, 2011), such as irritable bowel syndrome, inflammatory bowel disease, and diarrhea. The intestinal microbe-host interaction has become a research focus in microbiology (Kim et al, 2010). Exploring the potential regulatory role of probiotic candidates in intestinal epithelium is of great significance
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