Abstract
This study was conducted to evaluate the effect of dietary supplementation with Lactobacillus delbrueckii (LAB) on intestinal morphology, barrier function, immune response, and antioxidant capacity in weaned piglets challenged with lipopolysaccharide (LPS). A total of 36 two-line crossbred (Landrace × large Yorkshire) weaned piglets (28 days old) were divided into three groups: (1) nonchallenged control (CON); (2) LPS-challenged control (LPS); and (3) LAB+LPS treatment (0.2% LAB+LPS). Compared to the LPS piglets, the LAB+LPS piglets improved intestinal morphology, indicated by greater (P < 0.05) villus height in the duodenum and ileum; villus height : crypt depth ratio in the duodenum, jejunum, and ileum, as well as decreased (P < 0.05) crypt depth in the jejunum and ileum; and better intestinal barrier function, indicated by upregulated (P < 0.05) mRNA expression of tight junction proteins in the intestinal mucosa. Moreover, compared to the LPS piglets, LAB significantly decreased (P < 0.05) concentrations of TNF-α and IL-1β in the small intestine and increased (P < 0.05) IL-10 levels in the jejunum and ileum. Additionally, LAB increased (P < 0.05) T-AOC activities of the colon, GSH concentrations of the jejunum, and mRNA expression of CAT and Cu/Zn-SOD, while reduced (P < 0.05) MDA concentrations in the jejunum and ileum in LPS-changed piglets. Collectively, our results indicate that supplementation of LAB improved intestinal integrity and immune response and alleviated intestinal oxidative damage in LPS-challenged piglets.
Highlights
The maintenance of cellular redox homeostasis is imperative for the survival and normal function of cells
The excessive production of reactive oxygen species (ROS) or poor capability of scavenging reactive intermediates is considered causing a continuous imbalance in redox homeostasis, which resulted in endogenous oxidative stress [1, 2]
We established an oxidative stress model by injecting LPS-challenged control (LPS), in order to investigate whether Lactobacillus delbrueckii (LAB) administration could improve LPS-induced intestinal injury and health condition via regulating intestinal morphology, barrier function, immune response, and antioxidant capacity
Summary
The maintenance of cellular redox homeostasis is imperative for the survival and normal function of cells. Our team has proved the benefit of Lactobacillus delbrueckii (LAB, a kind of lactic acid bacteria) on improving the antioxidant status and stimulating the immune response in suckling piglets at the amounts of 1, 2, 3, and 4 mL at 1, 3, 7, and 14 d of age [16] Based on this finding, we hypothesized that LAB could ameliorate Escherichia coli LPS-induced intestinal integrity, immune function, and antioxidant capacity in weaned piglets. We established an oxidative stress model by injecting LPS, in order to investigate whether LAB administration could improve LPS-induced intestinal injury and health condition via regulating intestinal morphology, barrier function, immune response, and antioxidant capacity
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