Abstract
BackgroundProbiotics are widely used in intestinal microbiota imbalance caused by sepsis, however, the protective mechanism is still unclear. This study aimed to explore protective effect of Lacticaseibacillus rhamnosus TR08 on intestinal injury in septic mice.ResultsThe levels of serum inflammatory factors were reduced significantly in septic mice treated with L. rhamnosus TR08. The levels of sIgA in terminal ileum were significantly higher in probiotic treatment group than sepsis group. Intestinal pathological damage in septic mice improved and the expression of tight junction proteins increased after probiotic treatment. Sequencing of fecal microbiota showed that the abundance and diversity of probiotic treatment group were significantly better than those of sepsis group, and beneficial bacteria increased while some bacteria decreased in the phylum level.ConclusionL. rhamnosus TR08 could improve the integrity of intestinal barrier, enhance the intestinal mucosal immunity in septic mice, and rebalance the intestinal microecosystem.
Highlights
Probiotics are widely used in intestinal microbiota imbalance caused by sepsis, the protective mechanism is still unclear
L. rhamnosus TR08 improved the integrity of the intestinal barrier ELISA showed that the levels of TNF-α, IL-2 and IFN-γ were significantly higher in sepsis group compared with control group (P < 0.05)
Their levels were significantly lower than in sepsis group (P < 0.05) (Fig. 1). These data indicated that L. rhamnosus TR08 improved the integrity of the intestinal barrier in septic mice
Summary
Probiotics are widely used in intestinal microbiota imbalance caused by sepsis, the protective mechanism is still unclear. This study aimed to explore protective effect of Lacticaseibacillus rhamnosus TR08 on intestinal injury in septic mice. Sepsis is one of the important problems in critical care medicine [1]. More than 19 million people suffer from sepsis every year worldwide, with very high fatality rate [2, 3]. 3 million of patients who survive after sepsis have cognitive impairment [4]. Despite antibiotic treatment and surgical intervention, mortality rate of sepsis remains high [5]. Sepsis can be diagnosed when the sequential organ failure assessment (SOFA) rises by 2 points or more from the baseline in patients with infection or suspected infection [6].
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