Abstract
This study aimed to evaluate the efficacy of biofilms formed by lactic acid bacteria and Bacillus sp. (BLA) in preventing and controlling the formation of wild biofilms and/or planktonic forms of Salmonella Gallinarum (SG), Salmonella Heidelberg (SH), and methicillin-resistant Staphylococcus aureus (MRSA) on different surfaces. The SH and SG viability was evaluated in polystyrene plates, wood shavings, and soil samples. Two protocols were developed to examine the use of BLA in a preventive and control application. For analysis of Campylobacter jejuni (CJ) BLA was used only preventively in a polystyrene plate. Results showed that BLA was effective in preventing the growth of SG and SH in all matrices. The effectiveness of BLA for MRSA was lower than for SG and SH. The efficiency of BLA in preventing CJ growth seems to be related to the initial CJ contamination. BLA proves to be a potential alternative to control food-borne pathogens commonly encountered in animal production and food industry.
Highlights
Poultry production is one of the largest economic activities in the world
A biofilm could already be detected within 24h, but its distribution throughout the surface was more significant after 48h
The bacteria were stained with Propidium iodide and the matrix of the biofilms was stained with Calcofluor white (Sigma-Aldrich ®)
Summary
With affordable prices and practicality in preparation, chicken meat has convinced more and more consumers and is still gaining market share (ABPA, 2018). In this context of growing production and commercialization, quality control of poultry meat products and animal health requires stringent monitoring; birds’ carriage of food-borne pathogens is a crucial food safety concern for this industry. In addition to damage caused to animal and public health, these microorganisms lead to a significant negative financial impact (ABPA, 2018). Salmonellosis is one of the most complex zoonoses with the greatest impact on global public health and causes medical costs of up to $356 million per year (CDC, 2011). Enterica serovar Heidelberg (SH) from poultry production has recently been reported. SH has been proven to be difficult to control (Voss-Rech et al, 2015)
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