Lactic acid production by loofah-immobilized Rhizopus oryzae through one-step fermentation process using starch substrate.

Abstract

Rhizopus oryzae PTCC 5263 capacity in synthesis of lactic acid (LA) from 10g/l of soluble potato starch was determined using one-step fermentation process. Pellets were the favorable growing form of the free cells. The extent of the natural ability of the test fungus on biofilm formation on loofah sponge was examined by immobilizing R. oryzae (LIRO). The maximum LA concentration for the free cells and LIRO within 96h was 3 and 4g/l, respectively. In terms of specific starch utilization rate ([Formula: see text]) and specific LA formation ([Formula: see text]), LIRO performed more favorably compared to the free cells ([Formula: see text] and [Formula: see text]). Cell immobilization strategy was undertaken for the column reactor studies based on the statistically optimized levels of the inoculum size and temperature. Maximum production of the LA by the LIRO using an airlift reactor with net draft tube was 5g/l obtainable within 48h.