Abstract
Enterococcus faecium No. 78 (PNCM-BIOTECH 10375) isolated from puto, a type of fermented rice in the Philippines was used to produce lactic acid in repeated batch fermentation mode. Enzymatically liquefied sago starch was used as the sole carbon source, since sago (Metroxylon spp) is a sustainable crop for industrial exploitation. Liquefied sago starch was inoculated with E. faecium to perform the saccharification and fermentation processes simultaneously. Results demonstrated that E. faecium was reused for 11 fermentation cycles with an average lactic acid yield of 36.3 ± 4.71 g/l. The lactic acid production was superior to that of simple batch mode and continuous fermentation in terms of lactic acid concentration. An un-dissociated lactic acid concentration of 1.15 mM affected the productivity of the cells. Work is in progress to maintain and increase the usability of the cells over higher fermentation cycles.
Highlights
Lactic acid is an important commodity because it is a multi-functional versatile organic acid having a wide range of applications
In this regard the repeated batch fermentation (RBF) process combines the advantage of batch and fedbatch fermentation processes mainly making possible to conduct the process by long periods and improving the productivity compared to batch process (Treichel et al 2010)
Effect of sago starch in different forms on biomass production This experiment had the objective to justify the use of liquefied sago starch as carbon source for lactic acid fermentation
Summary
Lactic acid is an important commodity because it is a multi-functional versatile organic acid having a wide range of applications. One of the most important factors that affect the overall production cost in Lactic Acid Fermentation (LAF) is the raw material. On the other hand, Shibata et al (2007) reported the use of Enteroccus faecium 78 as a promising microorganism to produce L-(+)-LA directly from raw sago starch (RSS) in continuous culture by using a hollow fibre cartridge to recycle the cells. In their research the fermentation mode was of capital importance to enhance the productivity of the system In this regard the repeated batch fermentation (RBF) process combines the advantage of batch and fedbatch fermentation processes mainly making possible to conduct the process by long periods and improving the productivity compared to batch process (Treichel et al 2010). The main objective was to improve the productivity of the system, which includes the use of LSS, recycling of the yeast to speed-up the fermentation process
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