Lactic acid production and the inhibitory capability of lactic acid bacteria isolated from poultry feces

Abstract

This study was performed to isolate lactic acid bacteria (LAB) from poultry feces, identify the lactic acid production capability and its inhibitory effect against pathogenes as a promising probiotic candidate for livestock. Lactic acid bacteria were isolated and cultured on De Man, Rogosa and Sharpe (MRS) media. The parameters of this study are bacterial characterization and antimicrobial activity of LAB Isolates against gram-positive and negative bacteria. LAB characterization was performed by Gram staining test, morphological test, catalase test, and fermentation type test. Microscopic observation of LAB isolated by feces showed that cocci and bacilli morphologies. The gram staining test showed Gram-positive and Gram-negative test results and the catalase test showed all LAB isolates obtained were facultative anaerobic. The results indicated that LAB M1 isolates could produce greater lactic acid with more effective inhibitory capability against Staphylococcus aureus ATCC 25293 and Salmonella typhimurium FNCC 0050 compared to other isolates.