Abstract
Biosurfactants have a wide-range of applications due to their unique properties like specificity, not toxicity (from LAB) and relative ease of preparation. These properties hold promise of biosurfactants to increase breast milk benefit were isolated and described into Lactobacillus plantarum, Lactobacillus fermentum ,Lactococcuslactis, and Leuconostocmesenteroides.The degree of microbial destruction of disease, which promotes the effective remediation of disease spreading.This paper presents a review of available research, methods and publications regarding Biosurfactant extraction from Lactic Acid bacteria isolated from human breast milk. 3 samples of human breast milk was provided, LAB were isolated and described, Biosurfactants recovery and surface activity were tested and extracted endo and extra cellular. In other side 26 samples from eye patients were ordered, diagnosed and their sensitivity to biosurfactant were studied. The results showed that 5 isolates of LAB from human breast milk were biosurfactant producer but L. plantarum was the more efficiency in surface activity. In other side, out of 26 eyes sample 18 were positive to pathogenic bacteria included E.coli (7), Klebseilla (5), Staphylococcus aureus(3)and S.epidermides (3).Extracellular Biosurfactanthad good effect against tested bacteria, but endocellular (extraction by normal method) had not any effect against any bacteria, whereas by solvents method were the more active against all tested bacteria. The results are promising enough to continue the quest for enhancement of inhibition growth of pathogenic bacteria with biosurfactant application (extracted extracellular by solvents) to look forward for biosurfactant as a solution of antibiotic resistance problem. In this study we concluded thatL. plantarum was the more effectiveness in biosurfactant surface activity and the extracellular biosurfactant by solvent method for extraction were better than endocellular and normal method of extraction.
Highlights
Human breast milk consists of high amounts of necessary nutrients for infants, including carbohydrates, essential fatty acids, proteins, vitamins and minerals [1]
Five strains of lactic Acid Bacteria (LAB) were isolated from human milk, as shown in Table (1)and (2), according to results depicted in these tables, Lactobacillusplantarum (Lb. p.) and Lactobacillus fermentum (Lb. f.) were found and (1) strain of Leuconostoc (Le.), Lactocococcus was found in two species Lactococcuslactis 1 (Lc. l1) and Lactococcuslactis 2 (Lc. 12.) as shown in (Figure 1)
In other side of our study, 18 out from 26 samples that collected from eyes patients, were observed for bacterial presence., E.coli and Klebseilla were found in these samples in large number, Staphylococcus was found with two species S. aureusand S. epidermidis but in fewer number, 7 isolates were observed of E. coli and 5 isolates of Klebseilla, 3 isolates of Staphyllcoccusaureus and 3 isolates of S. epidermides, So we called them E. coli (1).to E. coli (7),Klebseilla(1)toKlebseilla(5), S. aureus(1)to S. aureus(3)and S. epidermides(1)to S. epidermides (3), to determine the activity of EX
Summary
Human breast milk consists of high amounts of necessary nutrients for infants, including carbohydrates, essential fatty acids, proteins, vitamins and minerals [1]. It plays an important role in development of infants due to transfer of microflora originated in breast milk [2]. More than 200 different species of bacteria have been described in human milk, like probiotics [5][6][7], including bifidobacteria, Streptococci and lactic Acid Bacteria (LAB) [8]. Biosurfactant is one of these products that produced by some species of LAB not all of them [16], and other microorganisms like Pseudomonas [17] ,and yeast [18] ,and can be defined as the surface-active biomolecules, with wide-range of applications, due to their unique properties like specificity, not toxicity and relative ease of preparation, these surface-active biomolecules have attached wide interest, due to their unique functional properties, biosurfactants were used in several industries including organic chemicals, mining, foods, cosmetics and many others [19]
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