Abstract
Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma. Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group). The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p<0,001). The increased activity of the examined biochemical parameters in the aqueous humour of the POAG patients points to the fact that other mechanisms, besides IOP, have a role in glaucoma pathogenesis.
Highlights
Glaucoma is a progressive chronic optical neuropathy
There were no significant difference in the tested parameters between men and BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2010; 10 (1): 84-88
The mean values of the all investigated parameters increased in older patients glaucoma and cataract subgroups, there were no differences between different ages inside two main groups
Summary
Glaucoma is a progressive chronic optical neuropathy. For a long time, this term has been equalized to increased intraocular pressure. The results of an oxidative stress are lipid peroxidation of cell membrane, the enzyme inactivation through sulph-hydrile groups, creating the proteins aggregation, polysaccharides polymerisation and the damage of nucleic acids It occurs in the chain of acute processes, such as in some inflammatory reactions. Α -hydroxybutirate dehydrogenase (HBDH) is an isoenzymes of LDH ( ) Extracellular activity of this intracellular enzyme increases under the condition of oxidative stress, due to the integrity of cell membrane destroying in the course of lipid peroxidation. This leads to an increase of lactic acid concentration, that is to say, of lactic acid salts. The Wilcoxon signed ranks test has been used for establishing statistic significance of the results
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