Abstract
The effect of chlorinated hydrocarbon insecticides upon rabbit-muscle lactate dehydrogenase was determined by both the direct determination of diphosphopyridine nucleotide reduction and a tetrazolium salt reduction method. A comparison of the two assay procedures revealed that the pronounced stimulation of lactate dehydrogenase LDH activity was noted with the tetrazolium salt method but could not be detected with a direct photometric determination method. Incubation of lactate dehydrogenase with DDT or heptachlor prior to the addition of either diphosphopyridine nucleotide or substrate resulted in a complete inhibition of enzyme activity. The inclusion of either diphosphopyridine nucleotide or substrate in the prior incubation mixtures afforded no protection to the enzyme.
Published Version
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