Laconic Review on Capillary Electrophoresis-Mass Spectrometry in Separation of Proteins

Abstract

Abstract: The separation technique known as electrophoresis is made possible by the ability of liquid molecules to travel through an electric field. Early in the 1980s, it was discovered that considerable separation efficiencies could be obtained by passing a voltage across a capillary. In the late 1980s and early 1990s, commercial capillary electrophoresis (CE) first became accessible. Mass spectrometry is a powerful tool for identifying unknowns, studying molecular structure, and investigating the fundamental principles of chemistry. The most efficient technique combines high-resolution separations by CE with high-sensitivity and selectivity detections by MS, which is typical for bioanalytical applications and targets or thoroughly high throughput studies of low-concentration analytes frequently occurring in complicated matrices. The combination of capillary electrophoresis with mass spectrometry, or CE-MS, has emerged as an effective standard technique for the analysis of a wide variety of analytes. CEMS, a proteomic technology that combines chromatographic techniques with MS to increase the resolving power, is a well-liked technique for proteome analysis.