Abstract
The inflammation of the reproductive system can affect reproduction causing partial or complete infertility. It is well known that lipopolysaccharide (LPS) triggers an inflammatory response in the whole organism, including immunologically privileged organs, e.g. the testicles. Adult male TCRalpha-/-, TCRdelta-/-, CD1d-/- and beta2m-/- on B10.PL (H-2(u)) and B10.PL control mice were intraperitonealy (i.p.) injected with lipopolysaccharide (LPS). The animals were killed 24h and 10 days post LPS treatment and their gonads were prepared for microscopic examination. Histological changes in the testes after LPS injection were found only in control B10PL and CD1d-/- mice. The experiments revealed disturbances in Leydig's glands structure, blood vessel dilatation in the interstitial tissue as well as degeneration of seminal tubule epithelium, disruption ofspermatogenesis and subsequent decrease of sperm cell number in the tubule lumen. These changes were noticed mainly 10 days after LPS treatment. Lack of either TCRalphabeta+ CD8+ or TCRgammadelta+ lymphocytes diminishes the response of testicular macrophages to LPS whereas the absence of CD1d-dependent NKT cells does not affect macrophage reactivity.
Highlights
TCRáâ+ CD8+ and TCRãä+ lymphocytes ameliorates LPS induced orchitis in mice preliminary histological observations
There is a decreased number of Leydig cells and macrophages in the interstitial tissue of these animals compared with the BALB/c strain (Fig. 1a)
Many host cells, including epithelial cells, macrophages, neutrophiles, natural killer cells, dendritic cells and macrophages belong to the first line of defense against infection by sensing conserved microbial structures called pathogen associated molecular patterns (PAMP) through Toll-like receptors (TLRs) (MEDVEDEV et al 2006)
Summary
TCRáâ+ CD8+ and TCRãä+ lymphocytes ameliorates LPS induced orchitis in mice preliminary histological observations. The experiments revealed disturbances in Leydigs glands structure, blood vessel dilatation in the interstitial tissue as well as degeneration of seminal tubule epithelium, disruption of spermatogenesis and subsequent decrease of sperm cell number in the tubule lumen These changes were noticed mainly 10 days after LPS treatment. Similar changes were observed in seminal tubules and Leydig’s cells These in vivo studies were fully confirmed by in vitro experiments showing that testicular macrophages exposed to LPS stimulate Leydig’s cells to inhibit testosterone production and suppress spermatogenesis (BRYNIARSKI et al 2004). At present it is not known how these changes proceed in mice which selectively lack different T cell populations. These data may suggest that macrophages are under negative regulation of different T cell populations
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