Lack of predictive value of reticulocalbin-1 and GST-Pai alterations for efficacy of postoperative adjuvant chemotherapy consisting of cisplatin plus vindesine in patients with p-stage IIIA-N2 non-small cell lung cancer (NSCLC) (JCOG9304A)

Abstract

7316 Background: We have been sought for molecular markers for prediction of prognosis or chemo-sensitiby to achieve “individualized therapy”. We detected a polypeptide (molecular weight 44.0 kDa) which significantly decreased in expression level in CDDP-resistant cells by comparing the two-dimensional polyacrylamide gel electrophoresis (2-DE) patterns of the parent strain and CDDP-resistant cultured lung cancer cells (H69 and PC14). The amino acid sequence suggested that this polypeptide might be homologous with reticulocalbin-1 (RCN-1). In our in-house cases with adjuvant chemotherapy using platinum-based drugs with positive expression of RCN-1 was a better outcome obtained compared with cases without RCN-1 expression. Methods: To evaluate which molecular markers would be define subset of patients who would be benefited from postoperative adjuvant chemotherapy. Results: Abnormal expression of RCN-1 and GST-Pai. was detected in 76% and 42%, respectively. There was no relationship between each molecular alteration and clinical-pathologic characteristics such as gender, age, or treatment arm. In addition, in none of patient subsets with or without above molecular markers, survival was significantly affected by postoperative adjuvant chemotherapy (RCN-1+P=0.2980, RCN-1-P=0.5873; GST-pai+P=0.7291, GST-pai-P=0.3398). Conclusions: Although the number of patients was small and our previous study suggested the reticulocalbin-1 molecule might have some kind of involvement in the chemical tolerance mechanism of platinum-based drugs, it is concluded that in this retrospective analysis these markers failed to predict survival or response to the adjuvant chemotherapy consisting of cisplatin and vindesine in NSCLC patients with N2 disease. No significant financial relationships to disclose.