Abstract
The effect of polyoma virus (PV) capsid proteins on mouse cell macromolecular biosynthesis was studied indirectly in vitro. The empty form of PV, separated on a cesium chloride density gradient, was mixed with purified mouse cell DNA prelabeled with 3H-thymidine. Two criteria were used to determine whether PV forms a complex with mouse cell DNA: (1) a reduction in the hemagglutinating activity of PV; and (2) the association of 3H-label with the precipitate of PV-anti-PV-serum, after the mixing of PV and mouse cell DNA. Our results indicate that PV does not form a complex with mouse cell DNA in vitro.
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