Lack of influence of the ACE1 gene I/D polymorphism on the formation and growth of benign uterine leiomyoma in Turkish patients.

Guldal Inal Gultekin,Rukset Attar,Seda Gulec Yilmaz,Turgay Isbir,A. Burak Dalan,Ozlem Timirci Kahraman,Ahmet Buyukoren,Hande Atasoy,Nazli Ucunoglu

doi:10.7314/apjcp.2015.16.3.1123

Guldal Inal Gultekin, Rukset Attar + Show 7 more

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https://doi.org/10.7314/apjcp.2015.16.3.1123

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Abstract

Uterine leiomyomas (ULM), are benign tumors of the smooth muscle cells of the myometrium. They represent a common health problem and are estimated to be present in 30-70% of clinically reproductive women. Abnormal angiogenesis and vascular-related growth factors have been suggested to be associated with ULM growth. The angiotensin-I converting enzyme (ACE) is related with several tumors. The aim of this study was to identify possible correlation between ULM and the ACE I/D polymorphism, to evaluate whether the ACE I/D polymorphism could be a marker for early diagnosis and prognosis. ACE I/D was amplified with specific primer sets recognizing genomic DNA from ULM (n=72) and control (n=83) volunteers and amplicons were separated on agarose gels. The observed genotype frequencies were in agreement with Hardy-Weinberg equilibrium (χ2=2.162, p=0.339). There was no association between allele frequencies and study groups (χ2=0.623; p=0.430 for ACE I allele, χ2=0.995; p=0.339 for ACE D allele). In addition, there were no significant differences between ACE I/D polymorphism genotype frequencies and ULM range in size and number (χ2=1.760; p=0.415 for fibroid size, χ2=0.342; p=0.843 for fibroid number). We conclude that the ACE gene I/D polymorphism is not related with the size or number of ULM fibroids in Turkish women. Thus it cannot be regarded as an early diagnostic parameter nor as a risk estimate for ULM predisposition.

Highlights

Uterine leiomyomas (ULM), are benign tumors of the smooth muscle cells of the myometrium
In we have compared the effects of the angiotensin-I converting enzyme (ACE) I/ D polymorphism in ULM patients and healthy controls in a Turkish cohort
It is well documented that the black race is more susceptible to develop ULMs compared to the white (Baird et al, 2003)

Summary

Introduction

Uterine leiomyomas (ULM), are benign tumors of the smooth muscle cells of the myometrium. They represent a common health problem and are estimated to be present in 30-70% of clinically reproductive women (Baird et al, 2003; Ekin et al, 2014; Tal and Segars, 2014). ULM are rarely associated with mortality; they may significantly affect the quality of life with abnormal uterine bleeding, menorrahagia, infertility, complication of pregnancy and pelvic pain (Ekin et al, 2014). Abnormal angionesis and vascular-related growth factors have been suggested to be associated with tumor growth (Tal and Segars, 2014). A clear pathogenesis, including a genetic pathway has not been described for ULM (Catherino et al, 2013). Several studies have shown that ULMs are influenced by genetics risk factors (Edwards et al, 2013), ; Hakverdi and colleagues (2013) recently discovered novel chromosome aberrations in ULM patients (Hakverdi et al, 2013)

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