Lack of induction of interleukin-2-receptor-α in patients with tuberculosis and human immunodeficiency virus co-infection: implications for pathogenesis

Abstract

Since expression of both interleukin-2 (IL-2) and IL-2-receptor-α (IL-2R-α) by lymphocytes is inhibited by human immunodeficiency virus (HIV) in vitro, we hypothesized that HIV-co-infection among persons with tuberculosis (TB) might impair T-lymphocyte responses to TB via this mechanism. We measured soluble IL-2R-α (sIL-2R-α), a surrogate marker of T-lymphocyte activation and proliferation, and soluble tumour necrosis factor receptor I (sTNF-RI) in sera from West African patients categorized into 4 groups: those with TB alone (TB+ HIV−, n = 55), CD4-matched groups with TB and HIV co-infection (TB+ HIV+, n = 50) or HIV infection alone (TB− HIV+, n = 35), and patients with neither disease (TB− HIV−, n = 35). The median level of sIL-2R-α was markedly greater in the TB+ HIV− group (1580 U/mL) compared to the TB− HIV− (670 U/mL; P < 0.001) and TB− HIV+ (880 U/mL; P < 0.01) groups. More importantly, the median concentration of sIL-2R-α was much lower in the TB+ HIV+ group (855 U/mL) compared to the TB+ HIV− group (1580 U/mL; P < 0.01) despite similar levels of sTNF-RI. These results suggest that T-lymphocyte activation in TB patients is impaired by HIV co-infection and, furthermore, this suppressive effect was independent of numerical depletion of CD4 lymphocytes. Impairment to IL-2-signalling might contribute to the profound impact that HIV has had on both the incidence and the clinicopathological manifestations of TB.