Lack of evidence for strand-specific inhibition of poliovirus RNA synthesis by 3-methylquercetin

Abstract

Buffalo Green Monkey cells were infected with poliovirus-1 in the presence of 3-methylquercetin or guanidine, and the formation of positive- and negative-strand viral RNA was monitored using single-stranded RNA probes. Both 3-methylquercetin and guanidine prevented the formation of plus-strand as well as minus-strand viral RNA, although, due to the high multiplicity of infection used, a high number of genomic viral RNA was always present in the cells.