Lack of effect of high-dose antioestrogen on the maturation and in-vitro fertilization of human oocytes.

Abstract

Thirty-four women requesting laparoscopic sterilization underwent a fixed schedule regimen for multiple follicular development which included norethisterone and clomiphene citrate. Follicle aspiration for oocyte recovery was attempted laparoscopically 34 h after administration of 5000 IU human chorionic gonadotrophin (HCG). Nineteen women were given 80 mg tamoxifen orally 4 h prior to HCG injection, while 15 acted as controls. There was no statistical difference in fertilization rates in vitro between tamoxifen-treated patients and controls (80 and 68% respectively). In addition, the morphological characteristics of the oocytes, the rates of cleavage, and the concentrations of oestradiol, progesterone and androstenedione in follicular fluid were similar in the two groups. Tamoxifen was detected in substantial amounts in follicular fluids of patients given tamoxifen. These results suggest that high-dose tamoxifen, in clinically used doses, does not adversely affect the final stages of maturation or the fertilization and early cleavage of human oocytes.