Lack of detectable immunoglobulin E receptor expression on 33 of 34 cell lines with natural killer-like or cytotoxic-T-lymphocyte activity

Abstract

We recently reported that a cloned murine cell line with “natural killer (NK)-like” cytolytic function and prominent cytoplasmic granules also expressed large numbers of plasma membrane receptors (Fc ϵR) which bound mouse immunoglobulin E (IgE) with high affinity (S. J. Galli et al., 1982, Nature (London) 298, 288). We have now performed IgE-binding studies with 31 additional cloned murine cell lines exhibiting “NK-like” lytic activity (defined as the ability to kill YAC-1 lymphoma cells) and three antigen-specific cytotoxic-T-cell clones. One of the NK-like clones expressed a small number of Fc ϵR ( 3.0 × 10 4 cell ) on one of the two occasions it was tested. None of the other clones, which were derived by several different approaches and which had a variety of surface glycoprotein phenotypes, expressed any detectable specific binding of mouse IgE. By contrast, mast cell clones consistently expressed large numbers of Fc ϵR. The expression of large numbers of high-affinity Fc ϵR would appear to represent a very uncommon characteristic of NK-like murine cell lines isolated under conditions similar to those described in this report.