Abstract
Problem statement: Glutathione S-transferase is super family genes th at encode enzymes which involve in the detoxification of the cell and protects DNA from damage. Conflicting results was found in several studies in association with GSTT1 and GSTM1 gene polymorphisms and prostate cancer. In this study, we determined the associatio n between the GSTT1 and GSTM1 gene polymorphism and prostate cancer in Iranian subjects as control with control subjects. Approach: Sixty number of sample were collected from both case and contr ol at Milad hospital, Tehran, Iran who were above 40 years of old. Genomic DNA was extracted from blood and the detection of GSTT1 and GSTM1 genotypes was done using multiplex PCR method. Results: The frequency of the null alleles in GSTT1 and GSTM1 was 15 and 41.66% respectively in prostate cancer patients and 21.66 and 56.66% respectively in control subjects. There was no asso ciation was found between the polymorphism and prostate cancer among Iranian subjects (p>0.05). Conclusion: This study failed to show an association with GSTT1 and GSTM1 gene polymorphisms and prostate cancer and has no susceptibility to Iranian prostate cancer subjects.
Highlights
Prostate cancer is one the most common cancer in men all over the world[1]
There was no previous record found in relation to GSTT1 or GSTM1 polymorphisms in Iranian subjects
A total of 120 Iranian subjects were recruited and the association was analyzed between the case and control subjects for the contrast of the null versus the non-deleted genotypes of GSTT1 and GSTM1 genes
Summary
Prostate cancer is one the most common cancer in men all over the world[1]. The incidence of prostate cancer in Asian countries is 2.3-9.8 cases in 100,000 persons-year[2], while in Iran it is 5.1 in 100,000 persons-year[3]. The latest global estimation shows that the incidence of prostate cancer in developing and developed countries was 5.3 and 19%, respectively[1] Both genetic and environmental factors could affect the risk of prostate cancer which seems to influence the metabolism of carcinogens[2]. Other factors such as diet and life style, there is strong evidence to show that genetic alterations play a major role in prostate cancer etiology[5] The Phase II metabolizing enzymes such as Glutathione S-. Several studies have been conducted to show the risk of developing prostate cancer can be affected by polymorphism of glutathione S-transferase[9]. The conflicting results initiated us to determine the presence/absence of GSTT1 and GSTM1 gene frequencies in Iranian prostate cancer patients and healthy individuals by multiplex-PCR method
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