Lack of association between the −1082 (A/G) IL-10 polymorphism (rs1800896) and spontaneous preterm birth in the Indonesian Acehnese population

Abstract

Abstract Introduction Preterm birth is associated with multiple mechanisms, and a contractile state during preterm birth is typically accompanied by a shift in signaling from anti-inflammatory to pro-inflammatory pathways. It has been hypothesized that a mutation within the promoter of the interleukin-10 gene (IL-10) that causes hypo- or hyper-production of IL-10 might be associated with spontaneous preterm birth. Aim To determine the association between the −1082 (G/A) single nucleotide polymorphism (SNP) of IL-10 and spontaneous preterm birth in Aceh, Indonesia. Material and methods A case-control study was conducted between June 2012 and July 2014 at Dr. Zainoel Abidin Hospital, Banda Aceh. A total of 40 preterm and 40 term births were included in the final analysis. Genotyping of the −1082 (G/A) IL-10 SNP was conducted using real-time polymerase chain reaction (RT-PCR) and was confirmed by sequencing. An enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of IL-10 in sera. The associations of the genotype distribution and allele frequency with IL-10 levels and preterm birth were assessed using the χ2 test. Results and discussion There was no association between the genotype distribution or allele frequency and the level of IL-10 in serum. There was also no association between the genotype distribution or allele frequency and spontaneous preterm birth. Conclusions There is no strong association between the −1082 (A/G) IL-10 SNP and spontaneous preterm birth in the Acehnese ethnic group.