Lack of association between CGRP-related gene polymorphisms and medication overuse headache in migraine patients

Masakazu Ishii ,Hirotaka Katoh ,Shunichi Shimizu ,Tatsuya Kurihara ,Ken-Ichi Saguchi ,Mitsuru Kawamura

doi:10.15761/imm.1000117

Abstract

We investigated whether calcitonin gene-related peptide (CGRP)-related gene polymorphisms are involved in the aggravation of migraines due to medication overuse. In total, 47 migraine patients (6 males and 41 females; 36.4 ± 10.3 years) and 22 medication overuse headache (MOH) patients (1 male and 21 females; 39.6 ± 9.9 years) who had migraine participated in this study. Calcitonin gene-related polypeptide-alpha (CALCA, α-CGRP, Insertion/Deletion rs1553005, rs145837941) and CGRP receptor (receptor activity-modifying protein 1: RAMP1, rs3754701, rs7590387) were analyzed by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (PCR-RFLP) methods. No significant differences were observed in the genotype distributions of CALCA and RAMP1 between migraine patients and MOH patients. The results of this study showed no association between CGRP-related gene polymorphisms and the complication of MOH in migraine patients.

Highlights

Migraine patients are prone to developing medication overuse headache (MOH) [1,2,3]
We previously reported that gene polymorphisms such as methylenetetrahydrofolate reductase and dopamine D2 receptor were associated with the complication of MOH in migraine patients [16], in addition to the tumor necrosis factor (TNF)-β gene polymorphism [20]
We focused on the relationship between calcitonin gene-related peptide (CGRP)-related gene polymorphisms and the complication of MOH in migraine patients

Summary

Introduction

Migraine patients are prone to developing medication overuse headache (MOH) [1,2,3]. The aggravation of migraines due to medication overuse needs to be prevented. Increasing CGRP through medication overuse seems to aggravate migraines. Sutherland et al [15] reported that CALCA polymorphisms (rs3781719, rs145837941) and CGRP receptor (receptor activity-modifying protein 1: RAMP1, rs3754701, rs7590387) are not involved in the pathogenesis of migraine. To the best of our knowledge, there have been no studies on the relationship between CGRP-related gene polymorphisms and MOH. We focused on CGRP-related gene polymorphisms such as CALCA and RAMP1 and investigated the relationship between CGRP-related gene polymorphisms and the complication of MOH in migraine patients

Methods

Results

Discussion