Abstract
A novel Gram-stain-positive bacterial strain, CHu50b-6-2T, was isolated from a 67-cm-long sediment core collected from the Daechung Reservoir at a water depth of 17 m, Daejeon, Republic of Korea. The cells of strain CHu50b-6-2T were aerobic non-motile and formed yellow colonies on R2A agar. The phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the family Microbacteriaceae, exhibiting 98.0%, 97.7% and 97.6% 16S rRNA gene sequence similarities to Glaciihabitans tibetensis KCTC 29148T, Frigoribacterium faeni KACC 20509T and Lysinibacter cavernae DSM 27960T, respectively. The phylogenetic trees revealed that strain CHu50b-6-2T did not show a clear affiliation to any genus within the family Microbacteriaceae. The chemotaxonomic results showed B1α type peptidoglacan containg 2, 4-diaminobutyric acid (DAB) as the diagnostic diamino acid, MK-10 as the predominant respiratory menaquinone, diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid as the major polar lipids, anteiso-C15:0, iso-C16:0, and anteiso-C17:0 as the major fatty acids, and a DNA G + C content of 67.3 mol%. The combined genotypic and phenotypic data showed that strain CHu50b-6-2T could be distinguished from all genera within the family Microbacteriaceae and represents a novel genus, Lacisediminihabitans gen. nov., with the name Lacisediminihabitans profunda sp. nov., in the family Microbacteriaceae. The type strain is CHu50b-6-2T (= KCTC 49081T = JCM 32673T).
Highlights
Since Park et al (1993) proposed the family Microbacteriaceae, 56 genera have been described validly in this family at the time of writing
During an investigation on iron and sulfur oxidizing microbial diversity in the sediment of a eutrophic freshwater reservoir (Jin et al 2017), a strain designated CHu50b-6-2T was isolated from the freshwater sediment of the Daechung Reservoir
Strain CHu50b-6-2T was recovered from a 67-cm-long sediment core (36° 220 3000 N, 127° 330 5800 E) collected from the Daechung Reservoir at a water depth of 17 m in Daejeon, South Korea. 1 g sediment sample was applied to serial dilution method
Summary
Since Park et al (1993) proposed the family Microbacteriaceae, 56 genera have been described validly in this family at the time of writing (http:// www.bacterio.net/; Parte 2018). 2000; Reddy et al 2003; Lee 2007; Kim et al 2008; Kim and Lee 2011; Shin et al 2011; Jang et al 2012; Park et al 2012; Schumann et al 2012; Jin et al 2013; Lai et al 2015). We describe the phylogenetic, genetic, phenotypic and chemotaxonomic characteristics of this novel strain, which is proposed to represent a new genus within the family Microbacteriaceae by using a polyphasic approach
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