Abstract
A method for ribose nucleic acid determination is described in details. This method is to be used on biological material of low desoxyribose nucleic acid content (moulds, bacteria). It is based on the hydrolysis of RNA by normal hydrochloric acid at 60° C during 10 minutes. Determination of RNA is made, on the supernatant fluid after extraction, by U.V. absorption in the 260 mμ band. During the hydrolytic process, 81% of the RNA are converted into nucleotides. Adenine and guanine are also liberated in smaller quantities. No other purine or pyrimidine compound has been detected.
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