Abstract
Cultures of Phanerochaete chrysosporium, operating with an inert carrier (nylon sponge) and a non-inert carrier (barley straw), were employed in order to study laccase production during semi-solid state conditions. Manganese (IV) oxide, added to the cultures increased laccase activity 16-fold especially in barley straw cultures, in which a maximum laccase activity of 360 U/l (one unit is defined as 1 μmol of 2,2‘-azino-di-[3-ethyl-benzothiazoline-(6)-sulphonicacid] oxidized per minute) was achieved.
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