Abstract
BackgroundEscalating energy security, burgeoning population and rising costs of fossil fuels have focussed our attention on tapping renewable energy sources. As the utilization of food crops for biofuel production culminates into food vs. fuel dilemma, there is an intensive need for alternatives. Production of biofuels from lignocellulosic biomass owing to its profuse availability and high holocellulose content is a promising area for research.ResultsIn the present study, pineapple leaf, an agro-industrial waste was pretreated with laccase to enhance the enzymatic digestibility of the substrate for improved production of reducing sugar. Variables determining enzymatic delignification of pineapple leaf waste have been optimized by response surface methodology based on central composite design. Maximum delignification of 78.57%(w/w) resulted in reducing sugar of 492.33 ± 3.1 mg/g in 5.30 h. The structural changes in pineapple leaf waste, after laccase treatment, were studied through Fourier transformed infrared spectroscopy, X-ray diffraction and Scanning electron microscopy. Specific surface area, pore volume, and pore diameter of the substrate were studied using the Brunauer–Emmett–Teller and Barrett–Joyner–Halenda methods and found a significant increase in the aforementioned parameters after delignification.ConclusionLaccase mediated delignification of pineapple leaf waste is a cleaner sustainable process for enhanced production of reducing sugar which can accomplish the demand for biofuels.
Highlights
Lignocellulosic biomass is a potential renewable resource for biofuel production due to wide availability, low cost and rich holocellulose content
For efficient utilization of holocelluloses for biofuel production, the lignin barrier should be distorted by laccase mediated delignification process
The breakdown of C=C, C–H bonds in lignin and C=O bonds in hemicellulose and lignin were observed (FTIR analysis), which might be the plausible reason for the decrease
Summary
Lignocellulosic biomass is a potential renewable resource for biofuel production due to wide availability, low cost and rich holocellulose content. In addition to these feedstocks, pineapple waste is another prominent lignocellulosics, which is being produced abundantly by canning industries throughout the world. The worldwide pineapple production was estimated to be 24.80 Mt from 1.02 Mha for the year 2013–2014 [5] the production from India being 1.74 Mt from 0.11 Mha [6]. The peel and stems of pineapple waste is employed for the production of valuable products like bromelain and vinegar [7]. Production of biofuels from lignocellulosic biomass owing to its profuse availability and high holocellulose content is a promising area for research
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
