Abstract
Magnetic poly(p-phenylenediamine) (PpPD) nanocomposite was synthesized via mixing p-phenylenediamine solution and Fe3O4 nanoparticles and used as a carrier for immobilized enzymes. Successful synthesis of PpPD/Fe3O4 nanofiber was confirmed by transmission electron microscopy and Fourier transform infrared spectroscopy. Laccase (Lac) was immobilized on the surface of PpPD/Fe3O4 nanofiber through covalent bonding for reactive blue 19 dye removal. The immobilized Lac-nanofiber conjugates could be recovered from the reaction solution using a magnet. The optimum reaction pH and temperature for the immobilized Lac were 3.5 and 65 °C, respectively. The storage, operational stability, and thermal stability of the immobilized Lac were higher than those of its free counterpart. The dye removal efficiency of immobilized Lac was about 80% in the first 1 h of incubation, while that of free Lac was about 20%. It was found that the unique electronic properties of PpPD might underlie the high dye removal efficiency of immobilized Lac. Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles. Immobilized Lac on PpPD/Fe3O4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; therefore, it provides an optional tool for dye removal from wastewater.
Highlights
Laccase (p-diphenol: dioxygen oxidoreductase, EC 1.10.3.2) belongs to the family of copper-containing oxidases, which catalyze the 1-electron oxidation of a wide range of inorganic and organic substances, coupled with a 4-electron reduction of oxygen to water [1,2,3,4]
Over a period of repeated operation, the dye removal efficiency was above 90% during the first two cycles and remained at about 43% after eight cycles
Immobilized Lac on PpPD/Fe3 O4 nanofiber showed high stability, easy recovery, reuse capabilities, and a high removal efficiency for reactive blue 19 dye; it provides an optional tool for dye removal from wastewater
Summary
Laccase (p-diphenol: dioxygen oxidoreductase, EC 1.10.3.2) belongs to the family of copper-containing oxidases, which catalyze the 1-electron oxidation of a wide range of inorganic and organic substances, coupled with a 4-electron reduction of oxygen to water [1,2,3,4]. Polyaniline and poly(p-phenylenediamine) nanofibers as supports for Lac immobilization has the following obvious advantages: (1) the high nanofibers have area a large area N–H and agroups high density of nanopores ontosuitable which enzymes can be specific surface andsurface functional of PpPD nanofiber are for the efficient efficientlyofabsorbed. These nanofibers are capable as polymer nanowires. We fabricated a magnetically separable PpPD/Fe3 O4 nanocomposite for Lac immobilization to increase enzyme loading, improve enzyme recovery and recycling, and create a surface microenvironment that permits electrical conductivity. The reuse capabilities of Lac and its removal efficiency for
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
