Laccase의 (+)-Catechin 기질을 이용한 Catechin-중합체 생성

Abstract

When the reaction condition of polymer production from (−)-catechin was examined, the catechin-polymer was produced, but the anti-oxidative activity was lower than that of (−)-catechin. Therefore, in this study, (+)-catechin, which has the highest content in green tea catechin, was used to establish the reaction condition for production of (+)-catechin-polymer having anti-oxidative activity by laccase. In addition, the absorption regions of supernatant and precipitates were compared using a scanning method in the UV-visible region (250~450 nm) to identify the presence of (+)-catechin-polymer along with HPLC analysis method. The optimal temperature and pH for laccase-induced (+)-catechin-polymer production were 30°C and pH 5.0. Also, the optimal (+)-catechin-polymer production was achieved at the 1.5% laccase and 0.5% substrate content. In substrate specificity of laccase, (+)-catechin and epicatechin were efficient, whereas EGCG and removed-GA were not. However, despite (+)-catechin-polymer production, it did not increase anti-oxidative activity as in the case of (−)-catechin-polymer, so we will try to find a suitable substrate among green tea catechins in the future.