Laboratory Tests for Blueberry Insect Control, 1996

Abstract

Abstract These tests were conducted using a BurkardR computer controlled spray apparatus to apply the materials at 10 lbs psi air pressure and an orifice setting of 6. For all applications, 0.2 ml test material was sprayed into each petri dish. For tests with the fungal pathogen, Beauveria bassiana, insects were sprayed in filter-paper lined, 10-cm diameter petri dishes; blueberry foliage was introduced immediately after spray application. For all other tests, blueberry foliage was placed in the petri dishes prior to application. Foliage was changed every 2-3 days. The number of insects ranged from 4-10 according to pest species and its developmental stage. Replicates ranged from 2-8 in number. Mortality of insects in test dishes was recorded at intervals of 1-5 days (see tables). Dead insects were held in separate petri dishes for up to 2 weeks to detect mycosis or parasitism. Log(dose)-probit (mortality) regressions were used to quantify the relationship between dose of test material and mortality. The date selected for performing the regression was usually 4 days after spraying, although the criterion used to select the date was evidence of significant mortality across the dosages.