Abstract

AbstractIn recent years, outbreaks of groundwater contaminated with pathogenic microorganisms have been reported worldwide. Monitoring the presence of pathogens in groundwater is highly important in order to assess the risks associated with a number of common practices, including water reuse and manure spreading. We conducted this study in laboratory-scale sand columns to compare the transport and retention of two E. coli strains, O157:H7 and JM109, in the subsurface environment. Bacterial breakthrough curves and deposition rates were obtained under various ionic strength conditions. The cell’s surface zeta potential and hydrophobicity were tested to characterize their surface properties. We also investigated the impact of Pseudomonas aeruginosa biofilm on the deposition of both E. coli strains in groundwater. The deposition behaviors of the E. coli strains could not be fully explained by extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory. Our results indicate the retention of E. coli O157:H7 in san...

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