Abstract
In experimental membranous glomerulonephritis (Mb-GN) mediated by homologous renal tubular epithelial antigen (Tub-Ag) -antibody complexes in rats, peculiar features were observed in comparison with heterologous immune-complex nephritis. Specifically, typical membranous type nephritis developed in rats with a persistently high level of autologous antibody and comparatively long periods were necessary for the development of renal lesions after the appearance of circulating antibody. To investigate this phenomenon in detail, the effect of combining of pertussis vaccine (PV) with Tub-Ag on experimental glomerulonephritis and the avidity of the autologous antibody to Tub-Ag were studied.An experimental Mb-GN was induced in Wistar strain rats with a single injection of homologous Tub-Ag which had been extracted from the kidney by pronase digestion. Some rats were injected with PV in combination with Tub-Ag. Avidities of the auto-antibody to Tub-Ag from nephritic rats and of the antibody to a heterologous antigen, bovine serum albumin (BSA), were estimated by the method of Farr with a slight modification.Serum level of antibody to Tub-Ag were higher in rats which had been given PV than in control rats. More severe morphological changes and a shorter incubation period were observed in the rats with high levels of antibody, indicating that the persistence of a high level of auto-antibody to Tub-Ag is important for progression of the renal lesion. Avidity of the auto-antibody to Tub-Ag from nephritic rats was markedly lower than that of the antibody to BSA.These results may explain some aspects of the peculiar profiles which were observed in this experimental Mb-GN mediated by homologous Tub-Ag.
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