Abstract
The euthanasia is one of the most studied, debated and controversial topics in the laboratory animal science area. All over the world, the ideal way to perform euthanasia in laboratory animals is sought, seeking, in summary, three main points, animal welfare during the procedure; minimal influence on the animals biological system, not to interfere with test results and accessibility and low cost. In Brazil, after the validity of Law No 11.794-2008, that regulated the use of animals for didactic and scientific purposes, structuring the Animal Experimentation Control Council (CONCEA). In its resolutions, CONCEA standardized the application of accepted euthanasia methods, mainly overdose of injectable anesthetics and restricted use of carbon dioxide (CO2).
Highlights
The laboratory animal science, an important scientific and technological development area in all countries, act, in summary, five sub-areas: Education, bioethics, legislation and biosafety in laboratory animals; Animal welfare and bio-models behavior; Technology for breeding animals in the house facilities; Manipulation and experimentation in biomodels and Building and management of the lab animal house facilities
The objective of our study was in accordance with national legislation, mainly through Council of Animal Experimentation Control (CONCEA) -RN 37 and DBCA of the Ministry of Science and Technology (MICT - Brazil) to evaluate the effectiveness of the proposed methods and which one would be most adequate for use, under our conditions, in mouse, to induce euthanasia in order to reduce the discomfort and suffering of the animal during the process, preserving animal welfare and the ease of handling and access of employees to perform the respective methodology [6,8,28]
Our results showed that the methodology that was most appropriate to the respective requirements was the association between Isoflurane and CO2 M, because through the ethogram the animal did not show any excitation or disorientation behavior, On the other hand, the use of injectable anesthetics, both Thiopental and the combination of Ketamine and Xylazine, presented a dynamic of death compatible with signs of discomfort and disorientation, making it impossible to assess the state of unconsciousness and the confirmation of death only occurred after 360 seconds
Summary
The laboratory animal science, an important scientific and technological development area in all countries, act, in summary, five sub-areas: Education, bioethics, legislation and biosafety in laboratory animals; Animal welfare and bio-models behavior; Technology for breeding animals in the house facilities; Manipulation and experimentation in biomodels and Building and management of the lab animal house facilities. In Brazil, the important milestone in laboratory animal science was reached, after 12 years in the National Congress, by the approval of the Law No 11.794, October 2008 (called Arouca Law) (BRASIL (a), 2008; CONCEA, 2020), regulated by Decree No 6.899 in the 2009 (BRASIL (b), 2008). Through this legal regulation, the National Council for Animal Experimentation Control (CONCEA, in Portuguese) was structured and standardizing of the Ethics Commissions on the Use of Animals (CEUAs) implantation to the each institution.
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