Abstract

Three techniques were compared for efficiency at extracting entomophilic rhabditoid nematodes from soil samples. The Baermann funnel extraction was superior to centrifugation flotation, and flotation sieving methods in recovering Neoaplectana carpocapsae Weiser dauerlarvae from sand with 71.2, 45.8, and 7.6% recovery rates, respectively. In a petri dish bioassay, larvae of the greater wax moth, Galleria mellonella (L.), were exposed to filtered Baermann funnel extracts of nematode-spiked field soil. This method was effective in detecting populations of entomophilic rhabditoids.

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