Abstract

The toxicological effects of some insecticide mixtures (chlorpyrifos, pyridalyl, chlorantraniliprole, emamectin benzoate, and methoxyfenozide), against the 3rd instar of Spodoptera larvae were evaluated. The results indicated that, LC50 values for each insecticide were 12.44, 0.88, 0.08, 0.16 and 3.35 mg L-1 respectively, and 0.458, 0.009, 0.0014 and 0.357 mg L-1 respectively.Also, the LC50 values of chlorpyrifos are increased by larvae weight increased. LC50 values of chlorpyrifos as a function of post-feeding initiation of Spodoptera larvae to pyridalyl, chlorantraniliprole, emamectin benzoate, and methoxyfenozide after two days (single exposure) were 1.33, 1.99, 0.74, and 0.37 mg L-1 respectively. Therefore, post-feeding initiation of larvae to previous insecticides tremendously decreased LC50 values of chlorpyrifos.Also, the percent inhibition of AChE from Spodoptera head capsules by chlorpyrifos, pyridalyl, chlorantraniliprole, emamectin benzoate, and methoxyfenozide (LC10 equivalent concentrations) were 44.5, 23.4, 19.4, 33.5, and 15.4%, respectively. The percent inhibition of AChE from Spodoptera head capsules by chlorpyrifos either pre-treatment or post-treatment with pyridalyl, chlorantraniliprole, emamectin benzoate, and methoxyfenozide were increased. This indicates that the toxic potential of these pesticides for the tested organism is greater when present in a mixture than in an individual.

Highlights

  • Cotton leafworm, Spodoptera littoralis (Boisduval), is one of the most serious and destructive polyphagous insect pest that lives on a wide range of crops (Su and Sun; 2014)

  • Pyridalyl, chlorantraniliprole, emamectin benzoate, and methoxyfenozide against 3rd instar of S. littoralis larvae were presented in table (1)

  • LC50 values of chlorpyrifos against pretreated 3rd instar S. littoralis larvae with pyridalyl, chlorantraniliprole, emamectin benzoate, and methoxyfenozide at LC10

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Summary

Introduction

Spodoptera littoralis (Boisduval), (family: Noctuidae) is one of the most serious and destructive polyphagous insect pest that lives on a wide range of crops (Su and Sun; 2014).

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