Abstract
Laboratory experiments using the acetylene blockage technique showed that denitrification rates in sediments from Lake Okeechobee, Florida, are described by Michaelis-Menten nitrate dependence and Arrhenius temperature dependence ( E act = 15.5 kcal, but do not appear to be affected by seasonal differences in the supply of carbon substrates in this eutrophic lake. Peak nitrate concentrations in the sediments are in the range of the half-saturation constant, and thus, for a given temperature, k d varies by a factor of approximately two over the typical range of nitrate concentrations. Most diagenetic sediment denitrification models employ a single-valued rate constant k d, to describe nitrate dependence. However, the use of a constant k d for eutrophic lake sediments may result in a significantly erroneous estimate of annual denitrification loss.
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