Laboratory diagnosis of heparin-induced thrombocytopenia in Asian Indians as investigated with functional and immunologic methods.

Abstract

Heparin-induced thrombocytopenia (HIT) was looked for in 33 Indian patients undergoing cardiovascular surgery who received unfractionated heparin (UFH). Platelet counts were performed before the initiation of UFH therapy and 5 to 16 days after administration of the therapy. A decrease in patients' platelet count > 35% of the baseline value or < 100,000/microL was considered to be suggestive of HIT syndrome. Heparin-induced platelet aggregation (HIPA) and enzyme-linked immunosorbent assay (ELISA) tests were performed in all the patients to detect antibodies against heparin/PF4 complex. Thrombocytopenia was found to be present in 10 (30%) patients. Of these, two patients were found to be positive as detected by HIPA and ELISA tests. These two patients were considered to have classic HIT syndrome. One of these had bleeding while the other was asymptomatic. Among the 23 non-thrombocytopenic patients and controls, HIT antibodies were not detectable by HIPA test in all but absent by ELISA in only 21 of them. Two of 23 non-thrombocytopenic patients had positive results on the ELISA test. Because the latter had negative test results on HIPA, they were considered to be false-positive results. Thus, HIT syndrome develops in approximately 6% of Indian patients treated with UFH.