Abstract
A laboratory ovipositional bioassay was developed to assess Brassica (Brassicaceae) germplasm for resistance, specifically antixenosis, to the cabbage seedpod weevil, Ceutorhynchus assimilis (Paykull). The effects of seedpod excision, cage size, seedpod length, weevil collection dates, male presence, seedpod to weevil ratios, and weevil preconditioning on C. assimilis ovipositional performance were evaluated. Standardized protocols were then used to assess the acceptability of Brassica napus L. and B. rapa L. germplasm to the weevil. Paired choice tests using B. napus as a standard (‘Bridger’) identified 1 resistant line, PI 176876 ( B. rapa ), and 2 susceptible lines, PI 469918 and PI 469849 ( B. napus ). These tests also confirmed that adult feeding punctures are not correlated with oviposition. In all cases, ovipositional preference was less pronounced in no-choice tests than in the paired choice tests. Results of 6 x 6 choice tests were consistent with the paired choice tests in assessing ovipositional preference.
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