Laboratory and field evaluations of oviposition responses of Aedes albopictus and Aedes triseriatus (Diptera: Culicidae) to oak leaf infusions.

Abstract

Organic infusions created by fermenting white oak (Quercus alba L.) leaves in water were evaluated as sources of attractant odorants and contact oviposition stimulants for gravid Aedes albopictus (Skuse) and Aedes triseriatus (Say). Infusions were bioassayed in the laboratory by giving single females a choice of ovipositing in 1 container with infusion and 7 containers with water. Ae. albopictus laid significantly more eggs in containers with infusion, regardless of concentration (dilutions ranging from 10 to 100%) or age (fermentation periods of 7, 28, 60 d), than in containers holding water. The largest proportion of eggs (76.8%) was deposited in response to a 60% concentration of 7-d-old infusion. In contrast, Ae. triseriatus exhibited variable oviposition responses but generally deposited the largest number of eggs in only a few concentrations of older age infusions. In binary "sticky screen" bioassays, there was no difference between the numbers of females attracted to infusion or water, indicating that oviposition responses to infusion were mediated by contact chemostimulants rather than by attraction to odorants. Oviposition responses to infusions by field populations of Ae. albopictus and Ae. triseriatus in Raleigh, NC, were evaluated with pairs of oviposition traps, one containing infusion and the other containing water. Generally, Ae. albopictus laid significantly more eggs in ovitraps containing infusion regardless of its age (7, 28, and 60 d old) or the mass of leaves fermented (126 g = 1x or 504 g = 4x) than in water. In contrast, Ae. triseriatus deposited an equivalent number of eggs in traps containing water or 1x, 80% infusion regardless of its age; however, the oviposition response to ovitraps containing 4x, 7-d-old, 50% infusion was significant. Placement of an automobile tire behind an ovitrap did not increase the number of Ae. albopictus eggs laid in ovitraps containing 4x, 7-d-old, 50% infusion or water relative to ovitraps without a tire. Our research indicates that baiting ovitraps with oak leaf infusion would increase the sensitivity of surveillance efforts for Ae. albopictus and Ae. triseriatus.