Laboratory Analysis of the Anti-Infectious Activity of Quantum Dots and Bioconjugates Based on Them against a Potential Eye Pseudomonas Aeruginosa Infection. Experimental Research (Part 4)

Abstract

This article is a continuation of a series of publications on the results of an experimental study on the possibilities of using quantum dots, as well as bioconjugates based on them as a promising treatment for inflammatory diseases of the eye. Of the whole variety of microorganisms, Pseudomonas aeruginosa (Pa) is the most formidable pathogen, leading to pronounced, sometimes fatal, changes throughout the body in general, and in the eye in particular, in connection with which it receives close attention from bacteriologists and specialists dealing with the treatment of pathologies caused by this microorganism. Now, the search for effective methods to combat this pathogen is one of the priorities of world health care.This article presents an analysis of the anti-infectious activity of bioconjugates based on quantum dots KTCdTe / CdMPA710 and KTInP / ZnSe / ZnS650 in synergy with III generation cephalosporin (Cefotaxin) against nosocomial Pa strains. Cultures of microorganisms, in the amount of 30 Petri dishes, were incubated in a thermostat at 350C for 18 hours (in the dark and under a source of photoexcitation). As a source of photoexcitation (the emission spectrum of the source corresponded to the absorption spectrum of QDs), we used an LED strip connected to an uninterruptible power supply battery placed in a thermostat. Evaluation of the effectiveness of the impact was carried out using the disk-diffusion method with the measurement of effective growth retardation zones (GRZ). According to the results of the study, it was revealed that the use of the obtained bioconjugate (QD + AB) significantly increases the ZZR.