Abstract
Objective Analysis the prevalence of rotavirus and norovirus in non-bacterial infectious diarrhea occurred in Fentai district of Beijing during November 2013 and character the rotavirus detected in these samples.Methods Forty-seven stool specimens were collected randomly from outpatients and 30 environment samples from consulting room of hopitals.Rotavirus and norovirus were screened by real-time RT-PCR.For those rotavirus positive samples,the VP4 and VP7 gene were amplified by RT-PCR.Sequence analysis of Vp7 and Vp4 gene was performed by using the software Mega 4.0.Results Thirty-seven cases had rotavirus infection with the same G9P [8] a serotypes.Only 2 cases were identified to have norovirus infection except 5 were rotavirus and norovirus coinfection.Rotavirus was found from 7 environment samples.Phylogenetic analysis demonstrated that VP7 genes in this study were highly homologous with that circulated in Beijing during 2010-2012.Conclusions The results indicated that rotavirus G9P [8] a was a major pathogen of this high incidence acute diarrhea.It is necessary to strengthen the surveillance of G9P[8] a in China. Key words: Rotavirus ; Sequence analysis
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