Abstract
Maleimidylsalicylic acid reacts with the Ca(2+)-ATPase of skeletal muscle sarcoplasmic reticulum with high affinity and inhibits the ATPase activity following a pseudo-first-order kinetic with a rate constant of 8.3 m(-1) s(-1). Calcium binding remains unaffected in the maleimide-inhibited ATPase. However, the presence of ATP, ADP, and, to a lesser extent, AMP protects the enzyme against inhibition. Furthermore, ATPase inhibition is accompanied by a concomitant decrease in ATP binding. The stoichiometry of the nucleotide-dependent maleimidylsalicylic acid binding is 6-10 nmol/mg ATPase, which corresponds to the binding of up to one molecule of maleimide/molecule of ATPase. The stoichiometry of maleimide binding is decreased in the presence of nucleotides and in the ATPase previously labeled with fluorescein-5'-isothiocyanate or N-ethylmaleimide A fluorescent peptide was isolated by high performance liquid chromatography after trypsin digestion of the maleimide-labeled ATPase. Analysis of the sequence and mass spectrometry of the peptide leads us to propose Cys(344) as the target for maleimidylsalicylic acid in the inhibition reaction. The effect of Cys(344) modification on the nucleotide site is discussed.
Highlights
The Ca2ϩ-ATPase of skeletal muscle SR1 catalyzes calcium transport coupled to ATP hydrolysis [1, 2], a process that is triggered by calcium binding
It is shown that the inhibition behavior of this maleimide is different from other known maleimides because it binds near the phosphorylation site, Asp351, inhibiting ATP binding but not the incorporation of phosphate, altering the relationship between the nucleotide-binding site and the phosphorylation site
We chose maleimidylsalicylic acid to inhibit the Ca2ϩATPase because it is similar in size to phenylmaleimide, which we studied previously [27]
Summary
The Ca2ϩ-ATPase of skeletal muscle SR1 catalyzes calcium transport coupled to ATP hydrolysis [1, 2], a process that is triggered by calcium binding. When the MSA concentration in the preincubation medium was 75 M, the ATPase inhibition occurred in a time scale of minutes, 75% inhibition being reached after 15 min of reaction (Fig. 1).
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