Abstract
High effective specific activity N-succinimidyl 4-[ 18F](fluoromethyl)benzoate was prepared using a reversed phase HPLC procedure. Reversed phase HPLC removed several additional impurities not removed by normal phase HPLC, thereby increasing the effective specific activity. Small amount (< 100 μg) of sensitive proteins such as erythropoietin can be labeled with this reagent in high yield without aggregation.
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